Fruit Lockers is a match three game. To make a match, use your mouse to swap two lockers so as to create a group of three or more. These groups can be in a straight line, an L shape or a box. This will cause the lockers to open, revealing their contents, usually a fruit. When you get a group of three fruits together, they will disappear and the empty lockers will drop down, shifting the position of the lockers above them. The fruit groups, like the locker groups, can be in a straight line, an L shape or a box. Fruits that drop down begin filling up the scale.
There are two play modes, normal and free. In normal mode, there is a time limit and, to end the round, you must fill the scale. In free mode, the scale never fills and there is no time limit.
Later in the game, you meet the giant spider. This creature will place a web on some lockers, whether they are open or not. A webbed locker or fruit cannot be moved or matched. At the time you meet the giant spider, you also earn brooms. These can be used to remove a web. You begin with three and can find more in some lockers. Later, you will find a hammer in some lockers. When you find the hammer, if you click it, the hammer will hit the locker it was in, dropping that locker as well as the lockers to the left, right, above and below that locker.
Farther along in the game, you will earn the Magic Key. This can be used to open any closed, web-free, unsealed locker. Use of the Magic Key is unlimited, however, in normal mode, it will cost you some of the fruit on your scale. In free mode, using the Magic Key will cost you points. You will also encounter sealed lockers. These cannot be opened or moved but are just barriers.