The new main menu
An overview of the ten challenges in the Challenge mode
Widow is one of the new spider-themed bosses.
To enter this room you need to suffer some damage, but it contains special chests.
Fighting spiders in a new room type. The webs slow you down.
Some of the devil rooms now have a more 'heavenly' theme along with the available bonuses.
The new final chapter
Judgement appears in a new form where you have to throw him hearts.
A room with the book Teleportation for Dummies
This new opponent can only be defeated by aiming for the hearts.
New boss screen
A level with the labyrinth curse where there are twice as many rooms (see map), including two boss rooms.
Isaac has now become the final boss.