Conga Bugs is a marble-removal game similar to Luxor. Bugs will conga along a path and, using your mouse, you must fire a bug into the line to create a bug of three or more sections. This bug will then disappear. You must keep clearing the line until there are no more bugs. After a conga line clears, the pusher bug at the end drops its hat. You can then collect this hat. Collecting enough hats garners you an extra life. Some bugs are carrying bug tokens. Collecting enough of these unlocks rare bugs that play instruments in the orchestra pit.
In later levels, you can collect power ups to help you. These may slow or stop the conga line or make it go in reverse for a short time. In between stages, you are shown comic panel stories that give some insight into insects.