Probably due to likeness rights, none of the actors from any of the five Star Trek shows and eleven films make a physical appearance in Star Trek Online. However, several characters who appeared as infants and young children do. Some include:
- Kirayoshi O'Brien, the infant son of Miles O'Brien from Star Trek: Deep Space Nine. Now grown and working for Starfleet at Memory Alpha.
- Naomi Wildman, the young child from Star Trek: Voyager. Now commanding Deepspace K7.
- Miral Paris, the daughter of Tom Paris and B'Elanna Torres from Star Trek Voyager. Now a Security Chief aboard the U.S.S. Kirk.
It should be noted however, that Leonard Nimoy and Zachary Quinto do appear as Spock, but in voice only.