Anna writes a book against her father's wishes, telling details about a dark sect he was once part of. After he is found murdered, she is assaulted by recurring nightmares about the cultists and their sinister rituals. She starts to investigate her family past with the help of two friends, traveling around the world to uncover the mystery surrounding the secret cult.
Bloodline of the Fallen: Anna's Sacrifice is a hidden object game where, most of the time, the objective is to find and collect all the items listed at the right side of the screen, on locations filled with assorted paraphernalia scattered around the scenery. In some scenes, the list shows a series of actions to be performed, or pictures of the objects that have to be found. The hint button sits below the list, showing the position of one of the required items when activated, but taking some time to recharge after use.
Mini-games take place on separate screens, where the player has to complete several challenges like clicking on sections of a snake drawing in sequence by following twisted arrow directions, finding a series of symbols on a book with a magnifying glass, spotting the differences between an object and its photograph, reassembling the torn pieces of a family portrait and finishing other similar tasks.