Let's Go Jungle: Lost on the Island of Spice is an on-rails shooter for one or two players.
Players take the role of a man and woman who are tourists stranded on an island which is overrun by giant insects. Using the cabinet mounted guns players must shoot their way through hordes of enemies, including giant spiders, swarms of insects, large dragonflies, frogs, piranhas and more.
The stages are called tourist attraction and they include "Jungle Safari", "River Cruise", "Elephant Ride" and "Island Hopping". Each stage ends with an end boss, including a giant spider, an enormous frog, a mutated plant and a big butterfly. Each boss has several hot spots displayed as targets which the players must shoot.
Occasionally the shooting action is broken up with quick time events where players must work together to perform synchronised actions. During the quick time events the players must duck to avoid obstacles, repeatedly tap the button to start an engine, or jump by pressing the buttons at the same time. At the end of each stage the players' compatibility is shown as a percentage in a heart shape.