Scooby and his friends are at the Coolsville Criminology Museum's grand opening when the Pterodactyl Ghost suddenly escapes with other monsters' costumes and yells revenge upon Scooby Doo and his gang.
The game features locations and characters from the motion picture. It includes sixteen levels which can be played in order (Story mode) or as the player wants (Arcade Mode). The player needs to gather clues, set traps, use disguises and avoid some scary monsters from the past. Game levels are based on actual movie stills and artwork.