The game features the original 4 turtles from the previous games in the series, as well as other fighters.
The game is very much in the tradition of the Mortal Kombat or Street Fighter series, pitting you against opponents one at a time. Some fighters (besides the turtles) will be familiar, such as the Rat King, or even Shredder (now called "Cyber-Shredder" here).
Though even people familiar with the turtles will see differences in them. They can now, for example, do moves involving projectiles, such as "fireballs".
The game's modes include a story mode, where you can only pick one of the turtles and go on fighting opponents in order to rescue April and Splinter, and a tournament mode, where you fight only for the fame and money.