Amazon Trail 3rd Edition is an educational quest up the Amazon river. The game begins in a museum where an Amazon artifact bearing the likeness of a jaguar comes to life and speaks to you. The jaguar implores you to explore the Amazon, learn all you can about it, and seek the wisdom of the ancient people. After this intro, you are teleported to Belém, port city at the mouth of the Amazon. You must select from among 4 guides, each offering unique qualifications. Pick up a supply package consisting of fish, trading packets, harpoons, and other necessities, and you embark to travel up the Amazon river.
Once along the river, your task is to navigate upstream and visit various points along the water to gather information and knowledge of the area. At the same time, you must manage your supplies and make sure you and your guide are provided for. Other activities include getting out of the boat and exploring the rainforest, where you can pick fruit to add to your supply pile, or snap pictures of the flora and fauna for your gallery. There is also a fishing mini-game where you can attempt to spear aquatic creatures with a harpoon from your supply stock in order to add to your food pile.